Summary: Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup. AQP embedded in proteoliposomes are exposed to an osmogenic gradient that triggers water flux. https://parisnaturalfoodes.shop/product-category/chlorella-spirulina/