Semi-quantitative RT-PCR Analysis was conducted to compare the consequences in the extraction protocols about the amplification of 3 prevalent housekeeping genes ( (B) Home made hot-begin Taq polymerase permits reaction set up at home temperature. BEARmix reactions were setup utilizing unmodified and very hot-start off (crosslinked) Taq polymerase with 20 https://www.facebook.com/NewTechFind/posts/pfbid032TPHc3JdtuJHT3gVQGc64WsvMnEpTWf7uC3nfkG55ojbcdzZaTURpZ191tmpeuPTl